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IGVFFI8090CHJY

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2692VBHQ(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW287_2_I2
md5sum
52041f18a645965813c5354965e343d6
Content MD5sum
89796beb36a17e0e4d9827d7b46b2246
File Size
880 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run1/2023-11-03/outs/fastq_path/AAC7TGLHV//LW287_S2_L002_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7TGLHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
I2
Average Read Length
10
Read Count
126874997

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW287) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated