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IGVFFI8077IAPC

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9976WMCI(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW236B-2_2_R2
md5sum
394f01d0730c30367bee955b17dcda1f
Content MD5sum
c7d34b49664553e6d80908e789de3997
File Size
625 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-03-24-McDermott-NextSeq.10X/2023-03-24/outs/fastq_path/AAC235NHV/LW236B-2_S6_L002_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC235NHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R2
Average Read Length
90
Read Count
21484076

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
783 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
4.5 GB
validated