IGVFFI8028SAKO

released

validated

File Set

IGVFDS8375BGSZ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR4_S362

md5sum

21f16df5ae8cdd18f7f87c7140f78a27

Content MD5sum

1e9ff11f88bc683ee9d2e7eae9bb10e4

File Size

7.7 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinB-PCR4_S362_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCCTCT:TGCG

Average Read Length

Read Count

330210

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7863HHJP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library