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INDEXED
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SequenceFile
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IGVFFI8012VJJP
IGVFFI8012VJJP
preview
validated
Json view
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS7288IQLC
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW434_5_I2
md5sum
53eff6f7650f38d56fba0385f93d17f8
Content MD5sum
4598d3178214b2e832c21662b233ef01
File Size
2.9 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon17_18/scRNA//LW434_S3_L005_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Sequencing Details
Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
5
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I2
Average Read Length
10
Read Count
462354564
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM8133YEXK
InVitroSystem
H9
H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW434), female, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library
Files Derived From This File
2 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI1539OEIK
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
667 MB
validated
IGVFFI5815NBHE
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
2.2 GB
validated
Attribution
Award
HG011996
Principal Investigator(s)
Bruce Posner
,
Gary Hon
,
William Kraus
,
Nikhil Munshi
,
Maria Chahrour
Contact P.I.
Gary Hon
Lab
Gary Hon, UT