{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI8008YPNQ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI8008YPNQ",
"aliases": [
"jay-shendure:CRISPRa_singlecell_iPSCneuron_VPH_sequence_CS1_10Xlane2_seqlane1_I1"
],
"assay_titles": [
"scCRISPR screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization"
},
"content_md5sum": "a21cf7126d79bc1857cece974a567cf1",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-09T00:51:03.848506+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS2220HHTN/",
"accession": "IGVFDS2220HHTN",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9913PXTT/",
"accession": "IGVFSM9913PXTT",
"classifications": [
"differentiated cell specimen"
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"term_name": "GM25256 (WTC-11)"
}
],
"summary": "GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
"file_size": 322906507,
"flowcell_id": "AAAMCTVHV",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI8008YPNQ/@@download/IGVFFI8008YPNQ.fastq.gz",
"illumina_read_type": "I1",
"input_file_for": [
"/matrix-files/IGVFFI8270PCLP/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 10,
"md5sum": "869b55f1c5454b461148b2a981ef745b",
"mean_read_length": 10,
"minimum_read_length": 10,
"read_count": 52547394,
"release_timestamp": "2024-08-13T20:47:22.569640+00:00",
"s3_uri": "s3://igvf-files/2023/12/09/53cf9d9c-8755-43be-9049-a9301221f5d6/IGVFFI8008YPNQ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NextSeq 1000/2000 P3 Reagent Kit",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0010963/",
"term_name": "Illumina NextSeq 2000"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_file_name": "/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/neuron_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAMCTVHV/CS1_2/CS1_2_S6_L001_I1_001.fastq.gz",
"summary": "I1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "53cf9d9c-8755-43be-9049-a9301221f5d6"
}