IGVFFI7967STOK

released

validated

File Set

IGVFDS7753HONE(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinE-PCR3_S113

md5sum

83352cf70f1590dc0df240505a8165e4

Content MD5sum

bb6a4675247bc9d9e2c0a9010a9d5681

File Size

11 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinE-PCR3_S113_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCACACGA:ATTG

Average Read Length

Read Count

475767

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9074NPMT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library