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IGVFFI7951DAQB

released
validated
File Set
IGVFDS9139TBCB(interference single-nucleus RNA sequencing assay (10x multiome with MULTI-seq))
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
ansuman-satpathy:igvf_exp10_rna_10x2_NGS1_r2
md5sum
4e05b91f9660e1c50f79729068b8a87d
Content MD5sum
fa79a2d289c269155d4ad3887c579340
File Size
22 GB
Submitted File Name
/oak/stanford/projects/igvf/Projects/230707_IGVF10_McGinnis/GEX/FASTQ/GEX_2_CMAS_IGVF10_R_S10_L004_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Flowcell ID
223JHYLT3
Lane
4
Sequencing Kit
NovaSeq X Series 10B Reagent Kit
Illumina Read Type
R2
Average Read Length
90
Read Count
517392579

Samples

1 item
Accession
Type
Sample Terms
Disease Terms
Summary
MultiplexedSample
multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
hdf5
raw feature barcode matrix
cell by gene in raw feature barcode matrix
Ansuman Satpathy, Stanford
275 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI7951DAQB, IGVFFI6948UZJO, IGVFFI7454QXNZ, IGVFFI7537RMDD
Ansuman Satpathy, Stanford
1.4 KB
validated

Attribution

Award
HG012076
Principal Investigator(s)
Contact P.I.
Ansuman Satpathy
Lab
Ansuman Satpathy, Stanford