IGVFFI7934CVHY

released

validated

File Set

IGVFDS6689ALTU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR4_S127

md5sum

5667d2faab1a920fd7640852e96f94aa

Content MD5sum

2615203a71336e0dc8cdce88a144cce8

File Size

35 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinA-PCR4_S127_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCGCCTT:ATTG

Average Read Length

Read Count

1472761

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2914OOTJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library