IGVFFI7918JLEC

released

validated

File Set

IGVFDS1748WPZO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139

md5sum

a748e4555704bb6d3225d1ba1c0f438a

Content MD5sum

8a3505ac2e408f3c58d28e4b8266a134

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinE-PCR1-Rep1_S139_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGCCTTG:TTGA

Average Read Length

Read Count

1096323

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2548FJWP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library