Home
Home
Search (⌘K or ⌃K)
Data
Methodology
Data Model
About
Help
Sign In
INDEXED
Home
/
SequenceFile
/
IGVFFI7867ZETP
IGVFFI7867ZETP
released
validated
Json view
File Set
IGVFDS6488UFGV
(gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
md5sum
7500a26401dd037c14720a41d2b584ae
Content MD5sum
89323bb02dcc91b6888f459c7fd0f502
File Size
40 MB
Submitted File Name
/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinA-PCRrep2_S193_R1_001.fastq.gz
Sequencing Details
Sequencing Platform
Illumina MiSeq
Sequencing Run
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
25
Read Count
1765508
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM4675JUYE
InVitroSystem
TeloHAEC
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford