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IGVFFI7852BFOU

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS0997MTQA(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW279B-3_2_R2
md5sum
a6c739ac4b31139cd7e6b4c9e2781abb
Content MD5sum
935cf3a177f55b57bdc885d62b67aa7c
File Size
613 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW279B-3_S25_L002_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7VLGHV
Lane
2
Illumina Read Type
R2
Average Read Length
90
Read Count
26342833

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
448 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
395 MB
validated