IGVFFI7847XCEY

released

validated

File Set

IGVFDS3773NYHJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR2_Rep2_S196

md5sum

03055a8d5187e107b373ee9c5d18a9ee

Content MD5sum

34a0e88be1abfc24050ad6d15e3a4c2c

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinA-PCR2-Rep2_S196_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCGCCTT:GCCA

Average Read Length

Read Count

965818

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9689GGGO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library