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IGVFFI7835NHIA

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS0922ZREC(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW421_7_R2
md5sum
c776d76e6ec514544ef8a6324616a38c
Content MD5sum
0f512b212a7ca1edb7e2130dc6d512f1
File Size
21 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/scRNA//LW421_S46_L007_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
7
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R2
Average Read Length
151
Read Count
276553107

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW421), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.9 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
513 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT