IGVFFI7780IFRA

released

validated

File Set

IGVFDS4704IOZJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR3-Rep1_S320

md5sum

1f91fa931818409c7c675bd7eb6bec41

Content MD5sum

af316935941c4e3bc4e5aaac74785a3e

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF2-BinB-PCR3-Rep1_S320_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CATGCTTA:ATTG

Average Read Length

Read Count

976154

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7955GCNG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library