IGVFFI7770QKUY

released

validated

File Set

IGVFDS8772YTOP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinF-PCR4_S108

md5sum

2c19d2a6482886c96b160bc66b66b449

Content MD5sum

fc8f18fb4e16e848a906399140021398

File Size

44 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinF-PCR4_S108_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTCGCTGA:ATTG

Average Read Length

Read Count

1875009

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9117BTSA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library