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IGVFFI7740IMWI

released
validated
File Set
IGVFDS2890JPBI(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinD-PCRrep12_I2
md5sum
437f108e4a72911de5f00deb1d07e624
Content MD5sum
a470d4670978ccdcd1faaf2c52c827e3
File Size
795 KB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinD-PCRrep12_S330_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
CCTTCGCA:TGAC
Average Read Length
4
Read Count
93037

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
6.7 KB
validated