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IGVFFI7738RMIS

released
validated
File Set
IGVFDS2884DTLZ(CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
richard-sherwood:18loci_uptake_Rep1_bottom40_read1
md5sum
762c155c0ea8fbc2ecef16e31c235956
Content MD5sum
ea9b12ee3dd26c1f5c9a82fe78cd4a2b
File Size
13 MB
Submitted File Name
/net/bgm/sherwood/Tian_demult_data/nextseq_051523_Tian/fixed/18loci_uptake_Rep1_bottom40_R1.fastq.gz
Checkfiles Version
v17

Sequencing Details

Sequencing Platform
Element Biosciences AVITI
Sequencing Run
1
Illumina Read Type
R1
Average Read Length
64
Read Count
510270

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
336 KB
validated

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital