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IGVFFI7701ZMBO

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS0373CFJS(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW438B_6_R2
md5sum
7bc4efc5d25ace99eef46555bb654ef9
Content MD5sum
6d73249d3eff1fc88b26a26e01f02c65
File Size
8.4 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW438B_S12_L006_R2_001.fastq.gz
Checkfiles Version
v17
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R2
Average Read Length
151
Read Count
155696719

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW438) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
674 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
2.0 GB
validated