IGVFFI7682XDAF

released

validated

File Set

IGVFDS6495PTGH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinA-PCR1_S37

md5sum

f93b41e11150dd28f7b3e48efc7e1187

Content MD5sum

f642ff3f8ecf0a9a2264962fb47e3f87

File Size

11 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinA-PCR1_S37_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AAGACACT:CTCT

Average Read Length

Read Count

470966

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1476GCQU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library