IGVFFI7647ERJF

released
validated
File Set
IGVFDS9310VIMF(gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
R1 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispra-ccr7-high-bin-rep3-R1
md5sum
b168162c31d6181507b48adb4979db0c
Content MD5sum
a8418b803f60b7e98ad7221ec8f196ee
File Size
29 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/CCR7pos-pSM54-TF-H3_S10_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
3
Flowcell ID
000000000-K4RT4
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
1245591

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
21 MB
validated

Attribution