IGVFFI7621INKU

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI7621INKU/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI7621INKU",
    "aliases": [
        "jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinC-PCR3_S111"
    ],
    "assay_titles": [
        "CRISPR FlowFISH screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "24c42ba7f0c299ed5ba13278390e8bc6",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-06-01T02:16:55.027578+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS5688OXJC/",
        "accession": "IGVFDS5688OXJC",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM7071BULA/",
                "accession": "IGVFSM7071BULA",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
                        "modality": "interference",
                        "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
                    },
                    {
                        "@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
                        "modality": "degradation",
                        "summary": "inactive AID system targeting RAD21"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002824/",
                        "term_name": "HCT116"
                    }
                ],
                "summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 19998267,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI7621INKU/@@download/IGVFFI7621INKU.fastq.gz",
    "index": "TCGGAATG:ATTG",
    "input_file_for": [
        "/tabular-files/IGVFFI5319EGKY/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 21,
    "md5sum": "ebd5b2dfa9286aa6a34a34f0d636b72b",
    "mean_read_length": 21,
    "minimum_read_length": 21,
    "read_count": 831298,
    "release_timestamp": "2024-07-10T20:14:27.782413+00:00",
    "s3_uri": "s3://igvf-files/2024/06/01/f7118058-6f0e-4230-8399-cf60bbb201b5/IGVFFI7621INKU.fastq.gz",
    "schema_version": "14",
    "seqspecs": [
        "/configuration-files/IGVFFI5915HQEK/"
    ],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0008565/",
        "term_name": "Illumina HiSeq 2500"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
        "title": "Benjamin Doughty"
    },
    "submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinC-PCR3_S111_R1_001.fastq.gz",
    "summary": "reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "f7118058-6f0e-4230-8399-cf60bbb201b5"
}