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IGVFFI7524SQKQ

released
validated
File Set
IGVFDS5892ADAN(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-FACS_Input-PCRrep2_I1
md5sum
69cb0b5c8e2ee77bf9b39afb8e65d640
Content MD5sum
3c8e63add977b82eab6fe26c50839611
File Size
961 KB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-BinAll-PCRrep2_S398_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
AGGATCTA:TTGT
Average Read Length
8
Read Count
108742

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford