IGVFFI7504AYGF

released

validated

File Set

IGVFDS3348ANJX(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR3-Rep1_S260

md5sum

7c08d1a53af31e206c2cf1e41988d9b1

Content MD5sum

03a6cc4fc13ad5147a20eacd3865cfa3

File Size

30 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF1-BinB-PCR3-Rep1_S260_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAACTCTC:GCCA

Average Read Length

Read Count

1358105

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4779RXHT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library