IGVFFI7499BCPY

released

validated

File Set

IGVFDS1748WPZO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145

md5sum

c3a6ae18d2857134f352c1481c2be629

Content MD5sum

920f12a41372454da83b63b38a885de8

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinE-PCR2-Rep1_S145_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTACGCAC:TTGA

Average Read Length

Read Count

1034273

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2548FJWP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library