IGVFFI7494YYGZ

released

validated

File Set

IGVFDS1293ORGQ(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-FACS_Input-PCRrep1_R2

md5sum

9ed529d8a230fdd275348cca7513af4d

Content MD5sum

9ce4db5f528d88943261c5e5b6d5079c

File Size

80 MB

Submitted File Name

220228pPPIF-FFSample-6-D21-pPPIF-Amp-pPPIF-2-1-1-BinAll_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 550

Sequencing Run

1

Illumina Read Type

R2

Index

GACAGTAA:TGAC

Average Read Length

150

Read Count

2049211

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford