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IGVFFI7374ZSED

released
validated
File Set
IGVFDS4371VPAL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep2-BinD-PCRrep1_I2
md5sum
2fd5dc9bd77bbe1006966bbaae20d9f4
Content MD5sum
4a5ef51a9c2b1971ca867f43a8f94b37
File Size
14 MB
Submitted File Name
220228pPPIF-FFSample-3-D21-pPPIF-Amp-pPPIF-1-2-1-BinD_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
ACTGTATC:TGAC
Average Read Length
4
Read Count
1954146

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford