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IGVFFI7335PJUM

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS6861GRJI(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW280B-3_1_R2
md5sum
2cf1714d0ed3004f9aa52f1f6be21359
Content MD5sum
969570111fa65ab1baa0c8ce6321f7b2
File Size
621 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW280B-3_S26_L001_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7VLGHV
Lane
1
Illumina Read Type
R2
Average Read Length
90
Read Count
26142704

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
448 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
395 MB
validated