IGVFFI7334BOPO

released

validated

File Set

IGVFDS4252ROMJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep2_S64

md5sum

9345aa6f22b283f1a62a4ba550639398

Content MD5sum

4e58592087b4d9daa8fc15d34e6eb506

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinC-PCR2-Rep2_S64_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACCAACTG:TCTC

Average Read Length

Read Count

1007064

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4577TQPO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library