IGVFFI7303TQKB

released
validated
File Set
IGVFDS6367YJDA(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinB-PCRrep2_I2
md5sum
308e4b81c21500fbf879d0a3612fa9ff
Content MD5sum
cc5df18ddfe5fde5b2e16ffa19190bb9
File Size
2.9 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinB-PCRrep2_S133_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
2
Illumina Read Type
I2
Index
TGCTCGAC:TGAC
Average Read Length
4
Read Count
376226

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution