IGVFFI7283SWVN

released

validated

File Set

IGVFDS7928JRLY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF2-BinA-PCR4_S163

md5sum

d17f9c26e05375027bc2b8d06aad9cd7

Content MD5sum

207cfaff5a4bad3949c7ea2d0e8ef2fd

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF2-BinA-PCR4_S163_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTAACATC:ATTG

Average Read Length

Read Count

775320

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8085CGHV

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library