IGVFFI7230YSEB

released

validated

File Set

IGVFDS2805FQAD(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7

md5sum

0f50403a4cc2d83687f7efb2f34d691f

Content MD5sum

9754ce5b11c9260958372374b372b590

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinA-PCR2-Rep1_S7_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCAGTTAG:TGCG

Average Read Length

Read Count

1100088

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3086DQMC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library