IGVFFI7228DOIR

released

validated

File Set

IGVFDS5497GDIR(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF2-BinE-PCR3-Rep1_S95

md5sum

70d15a352bb6c5308db56949f16a4431

Content MD5sum

0e9e1cf82ef428595a74f4a1c2cc9eb2

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF2-BinE-PCR3-Rep1_S95_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCCTTGGT:GCCA

Average Read Length

Read Count

939126

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1662MZZB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library