IGVFFI7218BQAT

released

validated

File Set

IGVFDS1648ZPEA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinB-PCR2_S32

md5sum

e8f5a9fc6af7e5cd49c5f054bee5f18d

Content MD5sum

48afd571f7ee56d7ae56ff9b52be3cc7

File Size

9.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinB-PCR2_S32_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCTAGG:CTCT

Average Read Length

Read Count

411790

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1489ABUX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library