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IGVFFI7211LNLZ

released
validated
File Set
IGVFDS0482NAKZ(gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
md5sum
fdd67fcf59718c42d3a391d8a92d1dd7
Content MD5sum
49676d038459b13c800547fbf36af6be
File Size
9.6 MB
Submitted File Name
/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinC-PCRrep1_S123_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina MiSeq
Sequencing Run
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
I1
Average Read Length
8
Read Count
1175684

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford