IGVFFI7202ZYZL

released

validated

File Set

IGVFDS4894APIT(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181

md5sum

3167f3757fe84bd752c9ad53d3ea7e8b

Content MD5sum

f7800b7d711a98747dfd06b0a31c09e6

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinD-PCR3-Rep2_S181_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AAGGATGT:GCCA

Average Read Length

Read Count

1231483

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0373CYTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library