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IGVFFI7148JTBN

released
validated
File Set
IGVFDS8929VNCP(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VP64_sequence_CS1_10Xlane2_seqlane2_R2
External Resources
md5sum
4d51143650f2c99bedde6f5377b1a34f
Content MD5sum
9b0209ca6d89ce63983011d8ea067eae
File Size
1.3 GB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VP64_CS1_2/VP64_CS1_2_S6_L002_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R2
Average Read Length
91
Read Count
40359874

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Files Derived From This File

3 items
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW