IGVFFI7085ICFZ

released

validated

File Set

IGVFDS0803KKZH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinF-PCR3-Rep1_S180

md5sum

8f395653dd9e47ec93e9cb7ea12cc4fd

Content MD5sum

7bbd113477745f4e5211121fe86ff1c8

File Size

42 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinF-PCR3-Rep1_S180_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATAGCGTC:ATTG

Average Read Length

Read Count

1950601

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4323ZIMC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library