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IGVFFI7063EJJV

released
validated
File Set
IGVFDS0182ZKUF(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinB-PCRrep2_R2
md5sum
deff3e4d87eed9c2158b58469a4fb67f
Content MD5sum
0485e049b22c3ef5282b82b331c4544a
File Size
17 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep3-BinB-PCRrep2_S112_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
2
Illumina Read Type
R2
Index
AGGATCTA:TGAC
Average Read Length
150
Read Count
380873

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford