IGVFFI7056URWW

released

validated

File Set

IGVFDS3673BXYO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR3_S125

md5sum

149991d87a07f59137168d8238334e3d

Content MD5sum

6e703be58eda0f0f79e0f9971db83345

File Size

16 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinE-PCR3_S125_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGCCTTG:ATTG

Average Read Length

Read Count

652096

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0596STHT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library