IGVFFI7043IHYQ

released
validated
File Set
IGVFDS2504PFZO(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep2_R2
md5sum
d14e3b86bb779c48a1ae74abd9ba3401
Content MD5sum
85c8ec683ed127c3cbb6f784c0eaa3d9
File Size
76 MB
Submitted File Name
220228pPPIF-FFSample-2-D21-pPPIF-Amp-pPPIF-1-1-2-BinD_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R2
Index
TCGCCTTG:TGAC
Average Read Length
150
Read Count
1943826

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution