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IGVFFI6800PCCO

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS6863PGGX(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW427_4_I1
md5sum
05521a812ffc01ffdd21d11b71152dcb
Content MD5sum
072eb6648e1510480627912d3effe333
File Size
3.1 GB
Submitted File Name
//project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon17_18/scRNA///LW427_S3_L004_I1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
4
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I1
Average Read Length
10
Read Count
594873563

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW427), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT