IGVFFI6765CFEW

released

validated

File Set

IGVFDS5647VZOO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF3-BinB-PCR3_S170

md5sum

e76fe5074170e2dba9c716641d5e7d66

Content MD5sum

def572f73099a85c32917509e1f4f448

File Size

30 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF3-BinB-PCR3_S170_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCCTCT:ATTG

Average Read Length

Read Count

1257991

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3595PQEE

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library