IGVFFI6736AYSD

released

validated

File Set

IGVFDS2251KSGP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR2_S19

md5sum

a51d70d9c8e7ea71bd725c6ff744dd87

Content MD5sum

311aedbb2f5aff589d9bebe2bc7cede3

File Size

10 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinA-PCR2_S19_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CATAGCGA:CTCT

Average Read Length

Read Count

435213

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8346JZPG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library