IGVFFI6708IHTH

released

validated

File Set

IGVFDS5076XXNM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinC-PCR2_S45

md5sum

38ca51000d32b53bd6737be4dad31e6b

Content MD5sum

3da43c691774616522357cc052f62b72

File Size

11 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinC-PCR2_S45_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCTCGGTC:CTCT

Average Read Length

Read Count

454094

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2659OCKX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library