IGVFFI6700LZGL

released

validated

File Set

IGVFDS8736DPTS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinC-PCR1-Rep1_S231

md5sum

30e84b0e39f7e81d65ac4f726b59edc6

Content MD5sum

e0201584f2bea165a20c57b162654a54

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinC-PCR1-Rep1_S231_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCCTAGCC:TTGA

Average Read Length

Read Count

938568

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2887QEDU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library