IGVFFI6670CCCW

released

validated

File Set

IGVFDS0887BEDU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin6hrs-FF2-BinC-PCR4-Rep1_S207

md5sum

2abe2686d3f557d29f9ec5a808f63e61

Content MD5sum

7060d88d321d7cfe4cb0f1dab5797fcb

File Size

6.2 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin6hrs-FF2-BinC-PCR4-Rep1_S207_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACAGGTAT:ATTG

Average Read Length

Read Count

283331

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5674HHJF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library