IGVFFI6659FRJR

released

validated

File Set

IGVFDS5620SSUW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR1-Rep1_S3

md5sum

c324d645c18d8950445e0f8b9167e21e

Content MD5sum

8e33f843fa5ab415a5af73ac8bb33e1b

File Size

26 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinC-PCR1-Rep1_S3_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCACATCT:TTGA

Average Read Length

Read Count

1207732

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7889BDAF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library