IGVFFI6643VIJQ

released

validated

File Set

IGVFDS1575SRMY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR3_S137

md5sum

4ea9e019d79c647f200164e6fe7d9632

Content MD5sum

b9d75ac842286de10d4053116e4b5e20

File Size

16 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinE-PCR3_S137_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCCTTGGT:ATTG

Average Read Length

Read Count

660820

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3377SBLH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library