IGVFFI6534BXXG

released

validated

File Set

IGVFDS6282WCIA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinE-PCR2-Rep1_S239

md5sum

02e90199aa24bb1617d5a497e5eeff03

Content MD5sum

d5dccc315115d7a49ed4f51e195d80d1

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinE-PCR2-Rep1_S239_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCCACAG:TTGA

Average Read Length

Read Count

838535

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0671URTU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library