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IGVFFI6515ZKTF

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2612LSTD(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW281B-3_1_I2
md5sum
93cac6e70158737b1e854518ff493ff4
Content MD5sum
07efae43c908e719a7750eecc211183e
File Size
182 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW281B-3_S27_L001_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7VLGHV
Lane
1
Illumina Read Type
I2
Average Read Length
10
Read Count
26510315

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW281), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
540 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
508 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT